Objectives

  1. To screen food ingredients, associated with high diversity microbiota in ELDERMET, in an artificial colon for ability to increase microbiota diversity.
  2. To establish base-line models of microbiota-inflammatory status in rodents conventionalized with low or high diversity microbiota.
  3. To translate in vitro findings of microbiota diversity-promoting food ingredients to pre-clinical models, and test dietary supplements for effect on microbiota diversity.
  4. To determine the effect of microbiota diversity on susceptibility to infection using in vitro and pre-clinical models
  5. To establish if differential effects upon the microbiota explain differential effects of lipids to cause obesity with or without inflammation in pre-clinical models.
  6. To establish if the inflammatory activity of lipid fractions can be mitigated by dietary interventions shown above to modulate microbiota diversity.
  7. To determine by intervention trial if specific dietary ingredients (established from tasks 1-5) can improve gut microbiota diversity in human subjects with a range of metabolic/inflammatory profiles.